Babin et al. 2022
Identification
BABIN, Thibaut, DEDOLE, Tommy, BOUVET, Pierre, MARCOUX, Pierre, GOUGIS, Maxime et MAILLEY, Pascal, 2022. Electrochemical label-free pathogen identification for bloodstream infections diagnosis: towards a machine learning based smart blood culture bottle [en ligne]. 17 octobre 2022. [Consulté le 15 septembre 2025]. Disponible à l’adresse : https://www.researchsquare.com/article/rs-2153621/v1
Potentiomètre low-tech pour suivre plusieurs flacons
La plateforme électrochimique multi-matériaux détecte la croissance bactérienne grâce à quatre types d’électrodes (polyaniline et Iridium oxide/carbon ink sensibles au pH et aux couples redox) déposées sur des circuits imprimés dans des flacons en polypropylène. Elles mesurent en continu le potentiel électrochimique (ELF) à 37 °C pendant l’incubation.
« By implementing a multi-material potentiometric platform in a blood culture bottle, we have developed a portable system for pathogen identification of Gram and of genus. »
« In this article, we investigate the use of potentiometric measurements in blood cultures, using a multi-material electrode array. The implementation of the electrode array into a blood culture bottle allows continuous monitoring of culture with minimal instrumentation. »
« We used 4 electrode materials based on commercially available inks that present differentiated electrochemical behaviour. Two materials were selected for their pH redox sensitivity, polyaniline and Iridium oxide/carbon inks. The third material exhibits an internal redox couple (Prussian blue) which is sensitive to redox metabolites. The latter electrode is composed of carbon and is essentially sensitive to the oxidation power of the environment. »
« The inks were deposited on printed circuit boards that are integrated in blood culture bottles. The potentiometric signal is recorded between the different working electrodes (WE) and the common reference electrode during incubation in blood cultures at 37°C under orbital motion. »